Our fluorophore-conjugated antibodies eliminate uncertainty and ensure both sensitivity and specificity, delivering dependable results. Rigorous quality checks and validations are performed to maintain consistency, enhancing the accuracy of your results. Our quality assurance process maximizes specificity while minimizing variability and ensures a high signal-to-noise ratio, whether you’re working with recombinant fluorescent antibody conjugates or eliminating free dye.
We offer a wide variety of fluorophores to help expand your research capabilities. Our selection includes Alexa Fluor dyes, fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridinin-chlorophyll-protein complex (PerCP), allophycocyanin (APC), and tandem dyes, enabling high-resolution, highly sensitive results.
The fluorophores listed below provide a broad range of excitation and emission spectra, enabling flexibility in various fluorescence-based applications:
• Alexa Fluor 405 (AF405) emits a violet color, with an excitation maximum (ExMax) at 402 nm and an emission maximum (EmMax) at 421 nm.
• Alexa Fluor 488 (AF488) produces a cyan-green fluorescence, with an ExMax of 495 nm and an EmMax of 519 nm, featuring a quantum yield (QY) of 0.92.
• Fluorescein isothiocyanate (FITC) also fluoresces in the cyan-green range, sharing the same ExMax of 495 nm and EmMax of 519 nm, with a QY of 0.92.
• Alexa Fluor 555 (AF555) emits a yellow-green fluorescence, with an ExMax at 555 nm and an EmMax at 565 nm, having a QY of 0.1.
• Phycoerythrin (R-PE) produces a yellow-green fluorescence, with an ExMax of 565 nm and an EmMax of 578 nm, featuring a QY of 0.84.
• Alexa Fluor 568 (AF568) emits an orange fluorescence, with an ExMax of 578 nm and an EmMax of 603 nm, with a QY of 0.69.
• Alexa Fluor 594 (AF594) produces an orange-red fluorescence, with an ExMax at 590 nm and an EmMax at 617 nm, featuring a QY of 0.66.
• Allophycocyanin (APC) emits a far-red fluorescence, with an ExMax of 650 nm and an EmMax of 660 nm, with a QY of 0.68.
• Alexa Fluor 647 (AF647) also emits a far-red fluorescence, with an ExMax of 650 nm and an EmMax of 668 nm, having a QY of 0.33.
• Peridinin-chlorophyll-protein complex (PerCP) fluoresces in the far-red range, with an ExMax of 482 nm and an EmMax of 675 nm.
• Alexa Fluor 680 (AF680) emits in the near-infrared (Near-IR) range, with an ExMax of 679 nm and an EmMax of 702 nm, featuring a QY of 0.36.
• Alexa Fluor 790 (AF790) produces a near-infrared fluorescence, with an ExMax at 784 nm and an EmMax at 814 nm.
Additionally, we provide custom antibody conjugation services to meet your unique labeling requirements. Our team of experts collaborates with you to create the optimal conjugate for your specific assay needs. Available labels include:
• Alexa Fluor dyes: 405, 488, 532, 555, 568, 594, 647, 680, 600, 750, 790
• PE, APC, FITC
• Biotin, HRP, Alkaline Phosphatase
• Gold nanoparticles (10, 20, 40, or 80nm)
• Oligonucleotide/DNA barcodes
Our Lightning-Link® antibody labeling technology simplifies conjugation with quick and easy-to-use kits for over 45 labels. Unlike traditional methods that can result in antibody loss and require time-consuming purification steps, Lightning-Link® enables direct labeling in just three simple steps. With only 30 seconds of hands-on time, researchers can efficiently conjugate antibodies without compromising yield or performance. Offering a broad range of labels and scalable options from 10 µg to 100 mg of antibody, our technology provides the flexibility needed for diverse experimental applications.
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Features
- We validate our primary conjugates in functional applications, ensuring accurate and reliable results. Our flow cytometry conjugates undergo additional ICC/IF testing to confirm signal localization.
- Our validated fluorescent-conjugated antibodies eliminate the need for time-consuming in-house validation, enhancing efficiency in your research workflow. This enables you to dedicate more time to advance your research and achieve your objectives.
- Our fluorescence-conjugated primary antibodies ensure high specificity and accuracy in your results. They minimize the risk of false positives by eliminating the need for a secondary antibody, delivering more reliable outcomes.
- Fluorescently conjugated primary antibodies labeled using our Lightning-Link® technology provide a simple, efficient, and loss-free solution for generating high-quality reagents, ensuring consistent and reproducible staining in fluorescence-based applications.
Applications
- Our Alexa Fluor dyes provide brilliant and photostable signals, ideal for immunofluorescence (ICC/IF), immunohistochemistry (IHC), and flow cytometry.
- Our Phycoerythrin (R-PE) offers unmatched brightness, ensuring exceptional sensitivity in flow cytometry.
- Our FITC (fluorescein isothiocyanate) is a versatile and cost-effective choice for ICC/IF, IHC, and flow cytometry.
- APC (Allophycocyanin) delivers deep, rich fluorescence, ensuring superior performance in flow cytometry.
What are fluorophore-conjugated antibodies?
Fluorophore-conjugated antibodies are antibodies linked to fluorescent molecules, allowing researchers to detect and visualize specific targets in biological samples using fluorescence-based techniques such as flow cytometry, immunofluorescence, and immunohistochemistry. These antibodies provide high sensitivity and specificity by emitting fluorescence when exposed to a specific wavelength of light, allowing researchers to analyze protein expression, protein localization, cell populations, and biomolecular interactions.
How are fluorescently labeled antibodies produced?
Fluorescently labeled antibodies are produced by chemically conjugating a fluorophore to an antibody through covalent bonding, typically using reactive groups such as amine, thiol, or carboxyl linkages. This process ensures stable fluorescence emission while preserving the antibody’s specificity and binding affinity, enabling accurate detection of target antigens in various fluorescence-based applications.
What factors should be considered when choosing a fluorophore?
When choosing a fluorophore, key factors include its excitation and emission wavelengths, brightness, photostability, and compatibility with the detection instrument. Factors such as spectral overlap, quenching effects, and the specific application (eg, flow cytometry, immunofluorescence, or multiplexing) also play a crucial role in the selection of the optimal fluorophore for accurate and reliable results.
How are fluorescently labeled antibodies stored?
Follow the specific storage instructions provided on the datasheet for your antibody. If no specific guidance is available, store the antibody according to the general recommendations provided here. Fluorescently labeled antibodies should be stored at -20°C, as there is no significant advantage to storing them at -80°C, with aliquots frozen and thawed only once, and any remaining antibody kept at 4°C. For short-term storage of one to two weeks, 4°C is acceptable, but it is important to follow the datasheet recommendations and use aliquots no smaller than 10 μL to minimize concentration changes due to evaporation and adsorption. Before aliquoting, mix the antibody well to ensure homogeneity and maintain performance.