Antibody Conjugation Kits
Our antibody conjugation kits offer a seamless solution for directly conjugating your chosen label to primary antibodies and proteins, ensuring efficiency and reliability. Available in four sizes - 3x10 μg (3 reactions, up to 30 μg each), 1x100 μg (1 reaction, up to 100 μg), 3x100 μg (3 reactions, up to 300μg each), and 1x1 mg (1 reaction, up to 1mg) - these kits provide flexibility and efficiency to meet your specific experimental needs. Label your antibody quickly and efficiently with no loss of material using our innovative antibody conjugation kits. Featuring our Lightning-Link® labeling technology, these kits provide a simple, quick, and reliable solution for antibody labeling, ensuring high efficiency and reproducibility.

Antibody conjugation, also known as antibody labeling, involves chemically linking an antibody to a specific tag, such as fluorescent dyes, enzymes, or metal isotopes, to facilitate detection in various applications. Traditional conjugation methods can be time-consuming and lead to antibody loss, requiring extensive post-conjugation purification steps.

Abcam antibody conjugation kits streamline the labeling process by removing the need for column separation steps, effectively addressing common challenges associated with this method. These include preventing material loss, avoiding sample dilution during column chromatography, minimizing batch-to-batch variability, and simplifying the scalability of experiments.

Our Lightning-Link® conjugation kits evolve on this process by enabling direct labeling with minimal hands-on time. Simply pipette your antibody into a lyophilized mixture containing the desired label and incubate for as little as 15 minutes (Lightning-Link® Fast range) or approximately three hours (Lightning-Link® range). This straightforward approach eliminates the need for  secondary antibodies, reducing protocol complexity and enhancing experimental efficiency.

These kits are available for over 45 label types. Explore our range of popular conjugation kits, designed to cater to your diverse research needs with precision and versatility. Designed to work seamlessly with our 13,000+ carrier-free antibodies, our antibody conjugation kits simplify experimental workflows, making them an essential tool for researchers in immunohistochemistry (IHC), flow cytometry, lateral flow assays (LFA), etc.

Features

Essential features

  • Streamlined and reliable:  Conjugate your antibody in just three simple steps, eliminating the risk of antibody loss associated with traditional post-conjugation purification methods.
  • Fast and convenient:  With only 30 seconds of hands-on time required, our Lightning-Link® antibody labeling kits let you focus on advancing your research.
  • Versatile and customizable:  Choose from a wide range of labels, including fluorescent dyes, enzymes, metal isotopes, and gold nanoparticles. Compatible with over 13,000 conjugation-ready carrier-free antibodies, these kits offer unparalleled flexibility for designing and completing your experiments.
  • Scalable and effective: Designed to handle 10 µg to 100 mg of antibody, these kits ensure consistent conjugation results, regardless of the scale of your experiment.

Applications

Applications

  • Used for FRET to study pH effects.
  • Applied in immunocytochemistry and in vivo imaging for imaging.
  • Utilized in flow cytometry for cellular analysis.
  • Used in ELISA for the rapid detection of your target.
  • Our HRP and alkaline phosphatase options are used to carry out enzymatic labeling.
  • Our fluorophores are used for fluorescent labeling.
  • Our tandem dyes ensure flexibility for advanced applications.
  • Utilized for nucleic acid studies.
FAQs

How does Lightning-Link® antibody conjugation work?

The Lightning-Link® process involves pipetting your antibody or biomolecule into a vial of lyophilized mixture containing the label of interest. Incubate from 15 minutes (Lightning-Link® Fast range) to around 3 hours (Lightning-Link® range) to create reproducible conjugates with no loss of antibody. This simple yet sophisticated protocol saves time and resources.

What makes Lightning-Link® technology unique?

This labeling technology eliminates traditional post-conjugation purification steps, preventing antibody loss. It offers a quick and easy solution with hands-on time as short as 30 seconds, ensuring scalability and consistent performance across experiments.

What are the key considerations for antibody conjugation?

For successful conjugation, ensure the antibody is purified at a concentration of at least 1 mg/mL and free from incompatible buffer constituents. For antibodies that do not meet these requirements, purification and concentration kits are available to help.

How is conjugation success confirmed?

Conjugation success can be checked using our conjugation check kits, which provide a quick and easy way to verify the labeling without needing specialized equipment. Alternatively, a trial experiment can confirm the conjugate’s performance in its intended application.