SpectraMax® i3x Multi-Mode Microplate Reader
The SpectraMax® i3x Multi-Mode Microplate Reader can perform a wide range of essential cell-based and biochemical assays using the included absorbance, fluorescence, and luminescence detection modes. SpectraMax microplate readers are the most cited and have advanced protein and cell biology research for over 40 years.
SpectraMax i3x multi-mode microplate reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime it grows with you as your research areas expand. For GMP/GLP regulated labs, the readers come equipped with our industry leading SoftMax® Pro GxP Software making us your partner in compliance
Unlimited breadth of user-upgradeable application modules expands research capabilities - SpectraMax® i3x Multi-Mode Microplate Reader
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
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Capture flash assays with ease
The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays.
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Reduce background noise
The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light.
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Increase fluorescence performance
Powerful combination of Xenon flash lamp and LEDs provides unmatched signal strength and superior sensitivity with Spectral Fusion™ Illumination.
Key Features of SpectraMax® i3x Multi-Mode Microplate Reader
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User-configurable applications
User-installable detection modules expand the reader’s detection capabilities to include AlphaScreen, time-resolved fluorescence, TR-FRET (including HTRF), fast kinetics with injectors, western blot detection, and more.
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No-stain cell analysis
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell analysis workflow
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Multi-channel functionality
Two fluorescence detection channels on the MiniMax 300 cytometer enable the analysis of cell viability or cell toxicity assays, including viability assays and transfection efficiency.
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Cellular pathway analysis
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
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Spectral Optimization Wizard
This built-in technology enables users to optimize their assay in a single-step by quickly identifying the best excitation and emission wavelengths.
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Auto Intensity Adjustment
Our patented technology acquires data over a wide dynamic range without the need to re-read the plate due to saturation effects. PMT gain adjustment is bypassed with the use of high-power LEDs as part of the Spectral Fusion Illumination.
| Item Height Range (Metric cm) | 32.5 cm |
| Item Width Range (Metric cm) | 39.2 cm |
| Microplate Reader Type | Multi-Mode Reader |
| Detection/Read Mode(s) | Absorbance, Fluorescence Intensity (Top/Bottom Read), Luminescence (Top Read), Time-Resolved Fluorescence (Top Read), Fluorescence Polarization (Top Read), Homogenous Time-Resolved Fluorescence (Top Read), Alphascreen (Top Read) |
| Detection/Read Type(s) | Endpoint, Kinetic, Spectrum Scan, Well Scan |
| Absorbance Read Time Range (sec) | 29 sec |
| Fluorescence Intensity Read Time Range (sec) | 22 sec |
| Luminescence Read Time Range (sec) | 21 sec |
| AlphaScreen Read Time Range (sec) | 22 sec |
| Fluorescence Polarization Read Time Range (sec) | 38 sec |
| Time-Resolved Fluorescence Read Time Range (sec) | 21 |
| Minimum Absorbance Wavelength Range (Metric nm) | 230 nm |
| Maximum Absorbance Wavelength Range (Metric nm) | 1000 nm |
| Minimum Fluorescence Intensity Wavelength Range (Metric nm) | 250 nm |
| Maximum Fluorescence Intensity Wavelength Range (Metric nm) | 850 nm |
| Minimum Luminescence Wavelength Range (Metric nm) | 300 nm |
| Maximum Luminescence Wavelength Range (Metric nm) | 850 nm |
| Fluorescence Intensity Sensitivity Top Read Optimization | Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well |
| Luminescence Sensitivity Top Read Optimization | Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well Cartridge: 20 amol ATP-flash |
| Time-Resolved Fluorescence Sensitivity Top Read Optimization | TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well |
| Fluorescence Polarization Sensitivity Top Read Optimization | FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well |
| AlphaScreen Sensitivity Top Read Optimization | AlphaScreen cartridge: <100 amol/well 384 well |
| Microplate Photometric Accuracy | < ±0.010 OD ±1.0%, 0 to 2 OD |
| Cuvette Photometric Accuracy | - |
| Photometric Precision | < ±0.003 OD ±1.0%, 0 to 2 OD |
| Microplate Reader Detector(s) | Silicon Photodiode, Photomultiplier Tube |
| Light Source(s) | High Power Xenon Flash Lamp, LEDs in Visible Range |
| Microplate Size Compatibility | 6 well plate, 12 well plate, 24 well plate, 48 well plate, 96 well plate, 384 well plate, 1536 well plate |
| Microplate Shaking Mode(s) | Linear, Orbital |
| Stray Light Measurement | - |
| Wavelength Selection | Filters, Monochromator tunable in 1 nm increments |
| Microplate Reader Validation Tool(s) | Absorbance Validation Plate, Fluorescence Validation Plate, Luminescence Validation Plate, Multi-Mode Validation Plate, SoftMax® Pro GxP Software |
| Includes Validation Tools | Yes |
| Includes Imaging Capability | Yes |
| Includes Injectors | Yes |
| Includes Western Blot Capability | Yes |
| Robotics/Automation Compatible | Yes |
| Includes Temperature Control Capability | Yes |
| Minimum Temperature Control Range Added to Ambient Temperature (Metric °C) | 4 °C |
| Maximum Temperature Control Range (Metric °C) | 45 °C |
| Item Depth Range (Metric cm) | 60.55 cm |