Scaling adoption of multiplexed flow cytometry to Unlock more Cellular Insights

Human-relevant models provide critical insights into molecular targets within living cells during drug candidate discovery. Live cell-based assays offer a systems biology approach to uncover the cellular mechanisms driving disease progression. However, the complexity of datasets generated by these advanced models is often challenging to analyze and interpret.

Researchers to comprehensively study human physiology via cellular interactions. Spectral flow cytometry is a new method that can help researchers multiplex their assays. Unlike conventional flow cytometry which measures specific wavelengths using discrete filters, spectral flow cytometry captures the entire emission spectrum of each fluorochrome. Owing to its ability to analyze multiple biomarkers within a single sample, spectral flow cytometry offers greater resolution and flexibility in experimental design. This is useful for experiments involving immune system profiling or studying disease progression through multiple tumor cell markers.

Modular solutions like the Beckman Coulter Life Sciences CytoFLEX Mosaic Detection Module make collecting and analyzing high-dimensional spectral data easier. This mosaic Detection Module connects to a Beckman Coulter Life Sciences CytoFLEX Flow Cytometer, enabling spectral flow cytometry. Spectral flow cytometry generates multiple fluorescent signals compared to conventional methods, requiring careful deconvolution and sensitive analysis to distinguish between signals. The module’s specialized software corrects overlapping spectra and isolates fluorescent signals, ensuring accurate marker identification. It also generates distinct spectral signatures for high autofluorescence, enabling the detection of dim and rare cell populations. These capabilities help reveal hidden patterns and insights in spectral flow cytometry data that are beyond the reach of conventional methods.

By integrating advanced analytical tools and innovative data analysis capabilities in their drug discovery journey, researchers can accurately interpret multiplexed experiment results, accelerating the progression of drug candidates to patients. Discover how the Life Sciences companies of Danaher can support your flow cytometry experiments with tailored solutions. Contact an expert today.